Description
The National Institutes of Health (NIH) is seeking a quotation for the acquisition of a 32-sample 10x Genomics Five Prime Single Cell Gene Expression and Immune Receptor Analysis Kit. The kit is required for high-resolution, single-cell analysis of a novel human minor salivary gland epithelial 3D model system. The kit must provide the capability to perform single-cell RNA sequencing, immune receptor sequencing, and identify clonally expanded B- and T-cell populations. The kit must also support high-throughput single-cell 5' gene expression profiling and paired V(D)J immune repertoire sequencing.
Key features of the required kit include:
• High-throughput single-cell 5' gene expression profiling
• Paired V(D)J immune repertoire sequencing for both T-cell receptors (TCR) and B-cell receptors (BCR)
• Sample multiplexing up to 32 samples in a single workflow
• Sufficient sensitivity, cell recovery, and library compatibility to identify clonally expanded B- and T-cell populations
• Compatibility with Chromium X instrument’s proprietary microfluidics, barcoding chemistry, and software