Description
The National Institutes of Health (NIH) is seeking to award a firm fixed-price contract for the provision of Single Cell Multiome kits. The kits will be used by the Center for Alzheimer's Disease and Related Dementias to study the molecular mechanisms underlying neurodegenerative disease. The required products include Chromium GEM-X Single Cell 3' Chip Kit v4, Dual Index Kit TT Set A, and GEM-X Universal 3' Gene Expression v4. The contractor must provide products that meet specific technical requirements, including compatibility with certain equipment and software. The contract will have a period of performance of 3 months, with a manufacturer warranty for one calendar year.
Key features of the required products include:
• Multifluidic chip for Gel Beads-in-Emulsion (GEM) generation
• Up to 40,000 cells per chip
• Support for multiple samples per chip
• Multilane chip enabling parallel GEM generation
• Compatibility with Chomium X, Chromium iX, Chromium Xo
• i7 + i5 dual index with each well containing a unique combination of i7 and i5 indices
• 96 unique dual index combinations per well
• Support for pooling of up to 96 samples per sequencing run/lane
• Compatibility with Chromium Single Cell 5’ workflows (GEX, VDJ, CRISPR) and GEM-X and Next GEM library prep kits
• 4-plex on chip multiplexing per channel for ~5,000 cells/samples or ~40,000 cells/chip
• 3’ gene expression (poly-A capture) with whole transcriptome coverage
• Cell barcode + UMI per transcript