NOI - Lonza 4D-Nucleofector Core Unit, X Unit, and

The National Institutes of Health (NIH) is seeking a sole source acquisition of a Lonza 4D-Nucleofector Core Unit, X Unit, and associated one-year warranty extensions. The equipment will be used to support research on virus-host interactions, including electroporation of primary cells to increase transfection efficiency for difficult gene targets. The required system must support Nucleofection of various cell numbers in multiple formats, process up to 32 reactions per minute, provide cell-type-specific optimized protocols for primary cells, guarantee genetic delivery to the cytoplasm and/or nucleus with no release of aluminum ions, and fit within a specific footprint. The delivery location is the Rocky Mountain Laboratories in Hamilton, MT. • The equipment must support Nucleofection of various cell numbers in multiple formats • The equipment must process up to 32 reactions per minute • The equipment must provide cell-type-specific optimized protocols for primary cells • The equipment must guarantee genetic delivery to the cytoplasm and/or nucleus with no release of aluminum ions • The equipment must fit within a specific footprint