Description
The Department of Veterans Affairs, Veterans Health Administration, Network Contracting Office 5, is seeking information from capable firms for market research purposes to identify potential sources for a Digital Polymerase Chain Reaction (dPCR) System. The system must meet certain minimum specifications, including the ability to perform absolute and accurate quantification without the need for standard curves or sample replicates, and detect down to 0.1% mutant allele frequency with a background of at least 10,000 copies of wild type target. The system must also utilize over 95% of a loaded sample across over 99% of available microchambers.
• The system must have a single instrument set up requiring under 10 minutes of hand time with samples.
• The system must be able to combine amplification and detection in a single reaction to eliminate post-PCR manipulations.
• The system must be able to complete 40 cycles of digital PCR using a fluorogenic 5´ nuclease assay in a digital PCR plate in under 90 minutes.
• The system must have optical channels, illumination, multiplexing, dynamic range, sensitivity, precision, sample throughput, and microchambers per reaction as specified.
• The system must have overall dimensions, weight, connections, cooling mode, power input, power rating, rated current, and maximum noise level as specified.