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  4. ›MERHAB- DCMF METABARCODING AND QPCR

U.S. Department Of The Interior·California

MERHAB- DCMF METABARCODING AND QPCR

Closes 5/27/2026$200k – $500kRequest for Quotation
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Description

The U.S. Geological Survey (USGS) California Water Science Center (CAWSC) requires various analyses of cyanobacteria DNA, including quantitative polymerase chain reaction (qPCR) and digital droplet PCR (ddPCR) analyses. The contractor will be responsible for extracting DNA from filter or biomass samples and performing qPCR and ddPCR analyses. The contractor must also perform amplicon metabarcoding on the 16S and 18S rRNA genes. The period of performance is from July 1, 2026, to September 30, 2027. Key aspects of the job include: • Extracting DNA from filter or biomass samples • Performing qPCR and ddPCR analyses • Performing amplicon metabarcoding on the 16S and 18S rRNA genes • Providing technical descriptions of the services being offered • Including a work plan, CV, references, and other necessary documents

Attached files

Contact

Elizabeth Adam(Contracting Officer)

[email protected]

(916) 278-9441

Key dates

Opened
5/21/2026
Questions due
5/25/2026
Closes
5/27/2026
Duration
14 months

Last updated

Details

Type
Request for Quotation
Industry
Environmental Services
NAICS
541380
Solicitation №
140G0326Q0123

Budget estimate

$200,000 – $500,000

The budget for this contract is not explicitly stated in the document. However, based on the scope of work and the requirements, it can be estimated that the contract value will be in the range of $200,000 to $500,000. This estimate is based on the assumption that the contractor will need to perform a significant amount of laboratory testing and analysis, which can be a costly process. Additionally, the contractor will need to have expertise in molecular methods applied to cyanobacteria and cyanotoxin production, which may also impact the cost.

AI estimate from the solicitation

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