Single Domain Antibody Development Service

The National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) is seeking a custom Single-Domain Antibody to the extracellular domain of the human Calcium-Sensing Receptor (CASR). The selected contractor will be required to deliver four clones of purified, validated, and 3XFLAG epitope-tagged sdAb (VHH), along with DNA minipreps and complete protein and cDNA sequence information. The project aims to enable the generation of a Variable Heavy-based injectable bioreagent for use in parathyroid tumor imaging and localization, as well as a potential radiopharmaceutical treatment for inoperable parathyroid cancer. Key features of the project include: • Delivery of 0.5 to 1 mg each of four clones of purified, validated, and 3XFLAG epitope-tagged sdAb (VHH) • Delivery of four DNA minipreps, corresponding to independent clones of sdAb (VHH) • Complete protein and cDNA sequence information for the above sdAb (VHH) • The project will involve several task areas, including sample submission, library panning, ELISA plate screening, and additional services.