USDA-SEA Long-Read Large Genome Sequencer

The United States Department of Agriculture – Southeast Area is seeking a Long-Read Large Genome Sequencer. The sequencer must be capable of sequencing reads up to a minimum of 20kb fragments and detecting 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) modifications in DNA sequences. The instrument must produce data in either FASTQ and/or BAM formats, and training in its use must be included with its purchase. The period of performance is not later than 60 days after award. • The sequencer must sequence larger genomes (i.e., animal) and produce 90 GB of sequencing data. • The sequencer needs to be able to sequence smaller fragments for other types of analysis like transcriptome studies. • Instruments must be able to detect 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) modifications in DNA sequences. • Data generated must be produced in either FASTQ and/or BAM formats. • Training in the use of the instrument must be included with its purchase and provide reagents for the training.