The United States Department of Agriculture (USDA) is seeking a contractor to perform low-pass whole-genome sequencing of approximately 576 cotton accessions from the U.S. National Cotton Germplasm Collection (NPGS-COT). The contractor will be responsible for receiving and inventorying genomic DNA samples, performing DNA quality-control assessments, preparing sequencing libraries, sequencing the accessions, performing standard sequencing quality-control procedures, and providing raw sequencing data files, quality reports, and sample manifests. The contractor must demonstrate experience supporting agricultural genomics and large-scale sequencing projects similar in scope and complexity to the U.S. National Cotton Germplasm Collection.
• The contractor must use the Revvity NEXTFLEX HT Whole Genome Sequencing library preparation system.
• The contractor must allow for direct communication between project personnel and sequencing staff.
• The contractor must provide a clear presentation of the required work and how it will be accomplished.
• The contractor must meet the required salient characteristics outlined in the statement of work document.
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