Notice of Intent to Sole Source: Grape Genome Sequencing Services (Req 1174046)

The USDA Agricultural Research Service’s Northeast Area Grape Genetics Research Unit requires a specialized, commercial genome‑sequencing service for unique wine and table grape accessions stored in the federal grape repository. The work includes high‑molecular‑weight DNA extraction with quality control, PacBio HiFi library preparation (including QC and size selection), and PacBio SMRT sequencing on a Sequel II platform to deliver approximately 20 Gb of HiFi data per accession. The contract is a firm‑fixed‑price sole‑source award to the University of Delaware’s Delaware Biotechnology Institute, covering a 12‑month base period with two optional 12‑month extensions, and an estimated total ceiling of $140,255.50. Interested parties may submit capability statements, proposals, or quotations, though the notice clarifies that competition is not guaranteed. • High‑molecular‑weight DNA extraction from grapevine tissue (challenging due to secondary metabolites) • PacBio HiFi library prep, QC, size selection • SMRT sequencing on Sequel II, ~20 Gb HiFi data delivery • 12‑month base period + two 12‑month option periods (potential 36‑month performance) • Sole‑source award to University of Delaware; total ceiling ≈ $140 K • Submissions accepted electronically via email (no specific deadline date provided)