Whole Genome Sequencing from Rainbow Trout blood samples

The USDA ARS National Center for Cool and Cold-Water Aquaculture (NCCCWA) requires whole genome sequencing to generate the data needed for De-novo genome assemblies from three rainbow trout blood samples. The contractor shall provide all services, supplies, equipment, and facilities necessary to extract high molecular weight (HMW) DNA from rainbow trout blood and provide sequencing of three genomic DNA samples. The scope of work includes PacBio sequencing, Illumina sequencing of whole-genome unamplified fragment libraries, and Hi-C sequencing. • The contractor must have experience in whole genome sequencing and genomics. • The contractor must be able to extract high-quality DNA from rainbow trout blood samples. • The contractor must be able to perform PacBio sequencing, Illumina sequencing, and Hi-C sequencing. • The contractor must be able to provide high-quality data and meet the requirements outlined in the scope of work.