DNA Extraction and Shotgun Metagenomics Sequencing of Fecal Samples

The National Institute of Health intends to negotiate a contract with Rush University Medical Center for DNA extraction and shotgun metagenomics sequencing of fecal samples. The contract requires the sequencing of 200 samples, including DNA extraction, library preparation, and shotgun metagenomics sequencing. The results will be used to understand cross-species validation of mechanisms related to bile acid- and FXR-induced increases in ketogenesis. The contractor will be required to provide raw data files to the PI, Dr. Katherine Maki. • The contract is for a sole source requirement, and the statutory authority for this requirement is 41 U.S.C. 253(c) (1) as implemented by FAR 6.302-1. • The contractor will be required to conduct shotgun metagenomics sequencing of fecal samples for 200 samples. • The contractor will be required to provide raw data files to the PI, Dr. Katherine Maki. • The contract is not a request for proposal, but a notice of intent to negotiate a contract with Rush University Medical Center.