R--SEAGRASS GENOME SEQUENCING

The U.S. Geological Survey (USGS) is seeking information on vendors capable of providing seagrass genome sequencing services. The services include DNA and RNA extraction, sequencing library preparation, sequencing, quality assurance, and electronic delivery of sequence data and technical documentation. The USGS is conducting research to assess genomic diversity and clonality in seagrass beds in Guam and surrounding Pacific islands for climate-resilient restoration. The research requires genomic and transcriptomic sequencing of marine seagrass plant tissues. • The contractor shall provide all labor, facilities, equipment, materials, sequencing platforms, reagents, quality assurance procedures, technical expertise, data processing, and electronic data transfer necessary to perform genome sequencing services for USGS-provided seagrass tissue samples. • The services shall include extraction of high-molecular-weight genomic DNA and high-quality RNA from flash-frozen seagrass leaves. • The contractor shall prepare high-fidelity long-read sequencing libraries from extracted genomic DNA and sequence them using the PacBio Revio platform. • The contractor shall convert extracted RNA to cDNA using the Iso-Seq Express 2.0 kit or an equivalent process suitable for long-read transcriptome sequencing. • The contractor shall deliver all final sequence outputs and a technical narrative identifying any issues, limitations, quality concerns, or deviations from expected methods.