Exosome Purification, Exosomal RNA Purification, and Small RNA-Sequencing Services

The National Institutes of Health (NIH) is seeking a qualified provider to perform laboratory services in support of a clinical research study. The services required include isolating intact exosomes, purifying exosomal RNA, and performing small RNA-sequencing on 31 plasma specimens. The contractor must furnish all labor, equipment, instrumentation, reagents, and materials necessary to complete these tasks while maintaining specimen integrity and chain of custody. The tasks required for this project include: • Purifying intact exosomes from 0.5 mL of plasma for each of 31 samples • Purifying exosomal RNA from intact exosomes and performing QC of the purified RNA • Performing small RNA-sequencing for 31 samples, including library preparation and library QC • Performing basic bioinformatics analysis and transferring all sequencing data to the client via hard drive and digital download.