Notice of Intent - Sole Source, SequalPrep Normalization Plates for DNA sequencing

The National Oceanic and Atmospheric Administration (NOAA) intends to negotiate and award a purchase order on a sole source basis with Life Technologies Corporation for SequalPrep normalization plates. These plates are used to standardize DNA quantity and remove low-quality short DNA fragments that can disrupt sequencing. The normalization plates must produce extracts within a specific concentration range of DNA quantity necessary for sequencing at the quality levels required by NOAA. The period of performance is anticipated to be for a twelve-month base period and three twelve-month optional ordering periods. During each twelve-month period, NOAA intends to order a quantity of twenty (20) normalization plates. • The plates are for use in next-generation sequencing protocols, including environmental DNA (eDNA), Genotyping-in-Thousands by sequencing (GT-seq), and whole genome resequencing. • The normalization plates must clean up and normalize the recovery of Polymerase Chain Reaction (PCR) reaction products in one process. • The resulting extract must be between 1.2 and 25 ng/μl of products per well within a 2- to 3-fold concentration range. • The plates must be delivered to 17109 PT LENA LOOP RD, JUNEAU, AK 99801.