The National Institutes of Health (NIH) is seeking a spectral flow cytometry system, specifically the Thermo Fisher Invitrogen Attune Xenith Flow Cytometer or an equal product, to support advanced immunophenotyping and functional immune cell analysis of patient-derived and preclinical samples. The system must meet certain minimum requirements, including spectral flow cytometry capability, six-laser optical configuration, high-parameter detection capability, acoustic-assisted hydrodynamic focusing technology, high-speed sample acquisition, compatibility with complex biological samples, automated sample loading, integrated instrument control and analysis software, touchscreen operation, and automated maintenance functions. The vendor must provide installation, operational startup, user training, and manufacturer-supported service coverage.
• The system must support spectral unmixing and conventional compensation workflows.
• The system must include a minimum of six excitation lasers, including ultraviolet (UV), violet, blue, yellow-green, red, and near-infrared (NIR) laser configurations.
• The system must include a minimum of 51 fluorescent detectors and 6 scatter detectors.
• The system must utilize acoustic-assisted hydrodynamic focusing technology.
• The system must provide high-throughput sample acquisition capabilities.
• The system must be capable of analyzing tumor, tissue, blood, and cellular therapy samples.
• The system must include compatibility with an automated sample loading system capable of processing both 96-well and 384-well plate formats.
• The system must include software for instrument control, data acquisition, spectral analysis, quality control monitoring, and data export.
• The system must include integrated touchscreen controls and automated maintenance features.
• The system must provide remote diagnostic capabilities and manufacturer-supported service coverage.
• The system must include the CytKick Max automated sample loading system.
• The vendor must provide installation, operational startup, user training, and manufacturer-supported service coverage.