NOI - Next Generation Sequencing Services for Detection of Somatic Genetic Mutations

The National Heart, Lung, and Blood Institute (NHLBI) intends to award a sole source purchase order to Genomic Testing Cooperative (GTC) for Next Generation Sequencing (NGS) services. These services will support clinical research by analyzing patient samples to detect somatic genetic mutations associated with bone marrow failure syndromes. The contractor shall provide Next Generation Sequencing services capable of analyzing a panel of approximately 302 genes associated with hematologic neoplasms and related conditions. Key features of the required services include: • Capability to analyze approximately 302 genes associated with hematologic disorders • Ability to isolate nucleic acids from multiple sample types (plasma, fresh cells, and FFPE tissue) • Massive parallel sequencing of coding DNA regions, including exons and flanking regions • Detection of mutations including insertions, deletions, and structural variants • Fragment length analysis capability for genes such as CALR, FLT3, and NPM1 • Ability to process low DNA input quantities • Secure delivery of results via electronic reporting system • Turnaround time of approximately 7–10 days after receipt of samples