B529--Prostate Cancer-Specific Sequencing Services

The San Francisco VA Healthcare System (SFVAHCS) is seeking a vendor to provide prostate cancer-specific sequencing services for a VA Merit award study under Dr. Franklin W. Huang, MD, Ph.D. The vendor will perform prostate cancer-specific sequencing for the SFVAHCS over a 3-year period, including minimum 30x and 60x coverage, FFPE-compatible workflow, tumor-normal matched sequencing, and secure data transfer method. The scope of work includes performing PCR+ Human WGS - 30x v3_FFPE_DRAGEN and 60x v3_FFPE_DRAGEN for 150 samples, ensuring anonymization and secure handling of all samples, and providing tumor and normal genome sequencing specific to prostate cancer tissue. Some key points to consider are: • The vendor must have a prostate cancer-specific sequencing protocol critical to the success of the study. • The vendor will work with previously collected tissue samples and prospectively collected samples from African American men undergoing radical prostatectomy. • The vendor must ensure sufficient data yield and quality for both the Whole Genome Sequencing (WGS) runs and downstream analysis. • The vendor must provide quality metrics, such as Q30 and coverage uniformity.