NextSeq1000 to NextSeq2000 upgrade.

The National Eye Institute (NEI) is seeking to upgrade its existing Illumina NextSeq 1000 Sequencing System to NextSeq 2000 capability. The upgrade will provide expanded sequencing flexibility and capacity by enabling the use of additional flow cell configurations and higher-throughput sequencing applications. The contractor shall provide all labor, materials, parts, equipment, software, travel, installation, testing, and technical services necessary to complete the upgrade and deliver a fully functional NextSeq 2000 instrument configuration. • The existing sequencing platform is manufactured by Illumina and is currently integrated into established laboratory workflows, sequencing protocols, software environments, and consumables. • The required upgrade is proprietary to Illumina and consists of the manufacturer-authorized NextSeq 1000 to NextSeq 2000 Upgrade, Part Number 20047256. • Compatibility with existing Illumina sequencing kits, reagents, hardware architecture, software, and validated research workflows is essential to support ongoing scientific studies and maintain operational continuity. • The contractor will perform the upgrade of the current NextSeq 1000 system to NextSeq 2000 capability and supply all parts and equipment required for the work. • The work will be done on the campus of NIH, Bldg 6, Rm 328, and is estimated to take 1 week to complete.